| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 751793 | Sensors and Actuators B: Chemical | 2006 | 6 Pages |
Abstract
With the help of chemical modification of silicon surfaces, FTIR-ATR was developed into a biosensing method to monitor biochemical processes on surfaces. In this proof-of principle work, FTIR-ATR crystals were covalently modified by oligonucleotides and two model systems were investigated, including recognition of anti-thrombin aptamer with thrombin and organic molecule binding to double helix DNA with sequence-specific photocleavage. The affinity of aptamer recognition and the affinity of photocleavage reagent towards double helix of DNA were quantitatively measured. Thus, it was shown that the proposed FTIR-ATR-based biosensing method allows for high-sensitive label-free detection of various biological systems.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wei Liao, Fang Wei, Dan Liu, Min Xie Qian, Gu Yuan, Xin Sheng Zhao,