Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7553440 | Analytica Chimica Acta | 2018 | 5 Pages |
Abstract
In this work, we presented a novel label-free biosensor for rapid detection of bleomycinsulphate (BLM). The biosensor was based on the fluorescent “turn off-on” of nitrogen-doped graphene quantum dots (N-GQDs), which was prepared in a green way from citric acid and ammonia. The richness of carboxyl groups on the N-GQDs enabled strong adsorption of ssDNA to the surface of N-GQDs through Ï-Ï stacking interactions, resulting in the effective fluorescence quenching of N-GQDs system. The ssDNA underwent an irreversible cleavage event via the oxidative effect of BLM with Fe(II) as a cofactor, thus a turn-on fluorescence signal was observed. Thereby, the concentration of BLM can be quantitatively determined in a broad range from 0.34â¯nmol/L to 1300â¯nmol/L with a detection limit of 0.34â¯nmol/L. The presented method was applied to the determination of BLM in human serum samples with satisfactory results.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiaotong Liu, Weidan Na, Qing Liu, Xingguang Su,