Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7553632 | Analytica Chimica Acta | 2018 | 29 Pages |
Abstract
Creatine kinase, a key biomarker associated with many debilitating physiological conditions has seldom been detected in biological fluids using functionalized gold nanoparticles (GNPs). We have developed a method based on the aggregation of cysteamine (Cys) functionalized GNPs in presence of ATP for effective detection of creatine kinase (CK-MM). Positively charged Cys-GNPs (brick red color) aggregate in presence of negatively charged ATP (blue color) but the process is prevented when CK-MM is added to the solution. The analytical response to the concentration of CK-MM is linear (R2â¯=â¯0.9850). The proposed method is selective in sensing the CK-MM for a range of 5.617â¯Ãâ¯103â¯ng/ml, 0.5617â¯ng/ml. The limit of detection was found to be 0.569â¯ng/ml in solution and 0.553â¯ng/ml in human serum with high selectivity.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Amit Kumar Sharma, Sunil Pandey, Yowan Nerthigan, Nandini Swaminathan, Hui-Fen Wu,