Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability

The immobilization method of physical adsorption combined with covalent crosslinking, which avoid the shortcomings of both the noncovalent and covalent coupling methods was developed. With the developed method, TYR was immobilized on Fe3O4-NH2, showing enhanced pH and temperature endurances, reusability, storage stability and higher affinity to substrate. Enzyme inhibitors were screened from 11 TCMs using the immobilized TYR.218