Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556120 | Analytica Chimica Acta | 2014 | 6 Pages |
Abstract
- The development of a FRET signal is modeled.
- Absorbance of acceptor and donor and KD of the complex are accounted for.
- The FRET efficiency and quenching are included as adjustable parameters.
- Software provided for fitting the parameters to the experimental data.
- Simulations can also be performed to study the impact of the various parameters.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shelly L. Casciato, Howard M. Liljestrand, James A. Holcombe,