Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7584320 | Food Chemistry | 2018 | 32 Pages |
Abstract
The presence of carcinogens in food is a major food safety concern. A nanocomposite-based electrochemical DNA biosensor was constructed for potential carcinogen detection in food samples by immobilizing amine terminated single stranded DNA onto silica nanospheres deposited onto a screen-printed electrode modified using gold nanoparticles. The effect of three different DNA sequences: 15-base guanine, 24-base guanine and 24-base adenine-thymine rich DNA on carcinogen (formaldehyde and acrylamide) detection was evaluated. The competitive binding of the DNA with the carcinogen and electroactive indicator, Methylene blue (MB) was measured using differential pulse voltammetry. Optimization studies were conducted for MB concentration and accumulation time, DNA concentration, buffer concentration, pH and ionic strength. Overall, the 24-base guanine rich DNA yielded the best results with a detection limit of 0.0001â¯ppm, linear range between 0.0001â¯ppm and 0.1â¯ppm and reproducibility below 5% R.S.D. Finally, the results obtained using the biosensor were validated using Ames test.
Keywords
Ammonium acetate (PubChem CID: 517165)Ethanol (PubChem CID: 702)Acetic acid (PubChem CID: 176)Sulfuric acid (PubChem CID: 1118)Electrochemical biosensorCarcinogensToxicityGold nanoparticlesSilica nanospheresPotassium dihydrogen phosphate (Pubchem CID: 516951)Potassium chloride (PubChem CID: 4873)Glutaraldehyde (Pubchem CID: 3485)
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nor Diyana Md. Sani, Lee Yook Heng, Radha Swathe Priya Malon Marugan, Nor Fadilah Rajab,