| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7629639 | Journal of Pharmaceutical and Biomedical Analysis | 2015 | 35 Pages |
Abstract
- An effective sample preparation protocol for cell metabolomics is developed.
- Fast and sensitive cancer cells metabolic profiling is obtained by CE-MS.
- Polyamines pathway examination corroborates the usefulness of the developed method.
- Ten metabolites are significantly altered by DFMO treatment in HT-29 cells.
Keywords
SAMDifluoromethylornithineS-adenosyl-l-homocysteineODCDFMOOrnithine decarboxylaseIAwSAHMTAGSSGPBSGSH3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideDMSOMTTPCAWaterS-adenosylmethionineCapillary electrophoresis-mass spectrometryChoPrincipal component analysisChinese Hamster OvaryEIEDimethyl sulfoxideMetabolomicsPhosphate-buffered salinePolyaminesreduced glutathionequality controlGABAoxidized glutathione
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Clara Ibáñez, Carolina Simó, Alberto Valdés, Luca Campone, Anna Lisa Piccinelli, Virginia GarcÃa-Cañas, Alejandro Cifuentes,