Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7704871 | Bioelectrochemistry | 2016 | 12 Pages |
Abstract
For this systematic review, 203 published reports on effects of electroporation using nanosecond high-voltage electric pulses (nsEP) on eukaryotic cells (human, animal, plant) in vitro were analyzed. A field synopsis summarizes current published data in the field with respect to publication year, cell types, exposure configuration, and pulse duration. Published data were analyzed for effects observed in eight main target areas (plasma membrane, intracellular, apoptosis, calcium level and distribution, survival, nucleus, mitochondria, stress) and an additional 107 detailed outcomes. We statistically analyzed effects of nsEP with respect to three pulse duration groups: A: 1-10Â ns, B: 11-100Â ns and C: 101-999Â ns. The analysis confirmed that the plasma membrane is more affected with longer pulses than with short pulses, seen best in uptake of dye molecules after applying single pulses. Additionally, we have reviewed measurements of nsEP and evaluations of the electric fields to which cells were exposed in these reports, and we provide recommendations for assessing nanosecond pulsed electric field effects in electroporation studies.
Keywords
TDRNsEPTime domain dielectric spectroscopyTl+TDDsPARPCyt CFWHMAMPKEGTAGFPFITCAMP-activated protein kinaseCa2 +MAPKROSEDTAethylene glycol tetraacetic acidEthylenediaminetetraacetic acidethElectroporationBAPTAtrypan blueTime domain reflectometryCellscytochrome cendoplasmic reticulumfull width at half maximumPlasma membranePhosphatidylserinefluorescein isothiocyanateSEMSystematic reviewscanning electron microscopeEthidium homodimerNanosecond electric pulsesgreen fluorescent proteinmitogen-activated protein kinasePropidium iodidepoly (ADP-ribose) polymerasepolyethylene glycolPEGReactive oxygen speciesCalcium ion
Related Topics
Physical Sciences and Engineering
Chemistry
Electrochemistry
Authors
Tina Batista Napotnik, Matej ReberÅ¡ek, P. Thomas Vernier, Barbara Mali, Damijan MiklavÄiÄ,