Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7704969 | Bioelectrochemistry | 2016 | 9 Pages |
Abstract
The electroporation (EP) supports various types of anticancer therapies by the selective transport of cytostatics. Increase in intracellular calcium level by EP may be a new approach to fibrosarcoma treatment. Calcium is one of the most important factors of cell proliferation, differentiation and cell death (apoptosis or necrosis). Calcium level balanced by electroporation can cause different effects on normal and pathological cells. The efficiency and safety of electroporation combined with Ca2Â + ions were examined in our study. The two muscle cell lines were used: normal rat skeletal muscle cells - L6 and cancer muscle cells - Wehi-164 (fibrosarcoma). Two CaCl2 concentrations were tested: 0.5Â mM and 5Â mM combined with EP parameters: 1000Â V/cm, 1200Â V/cm, and 1500Â V/cm. The results show that EP supported by Ca2Â + is cytotoxic for Wehi-164 cells and simultaneously safe for normal muscle cells. The main type of cell death - apoptosis - was confirmed by Tunnel and Annexin V/PI assay. Additionally, sPLA2 pro-tumorigenic influence was proved by immunocytochemistry. Moreover, EP with 0.5Â mM of Ca2Â + slightly stimulates the normal muscle cells - L6 to increase proliferation.
Related Topics
Physical Sciences and Engineering
Chemistry
Electrochemistry
Authors
Anna Zielichowska, MaÅgorzata Daczewska, Jolanta Saczko, Olga Michel, Julita Kulbacka,