Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7705218 | Bioelectrochemistry | 2015 | 6 Pages |
Abstract
This work presents a sandwich-type electrochemical impedance immunosensor for detecting Clostridium difficile toxin A (TcdA) and toxin B (TcdB). Single domain antibody conjugated gold nanoparticles were applied to amplify the detection signal. Gold nanoparticles (Au NPs) were characterized by transmission electron microscopy and UV-vis spectra. The electron transfer resistance (Ret) of the working electrode surface was used as a parameter in the measurement of the biosensor. With the increase of the concentration of toxins from 1Â pg/mL to 100Â pg/mL, a linear relationship was observed between the relative electron transfer resistance and toxin concentration. In addition, the detection signal was enhanced due to the amplification effect. The limit of detection for TcdA and TcdB was found to be 0.61Â pg/mL and 0.60Â pg/mL respectively at a signal-to-noise ratio of 3 (S/NÂ =Â 3). This method is simple, fast and ultrasensitive, thus possesses a great potential for clinical applications in the future.
Related Topics
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Authors
Zanzan Zhu, Lianfa Shi, Hanping Feng, H. Susan Zhou,