Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7709416 | International Journal of Hydrogen Energy | 2017 | 8 Pages |
Abstract
This work presents the integration of a paper-based microfluidic fuel cell inside a lateral flow assay-human immunodeficiency virus (HIV) test. To modify the HIV test, a bioanode that oxidizes the glucose present in a serum or blood sample and a cathode that reduces the oxygen delivered from air were stacked on the bottom and top, respectively, of a paper-strip test. A mixture of glucose oxidase enzyme, Nafion®, glutaraldehyde, deionized water and tetrabutylammonium bromide were deposited on a methylene blue electro-polymerized Toray® paper and used as the bioanode. Meanwhile, Pt/C (Vulcan XC-72) on Toray® paper was used as an air-breathing cathode. The GOx enzyme anchored on the carbon-based surface was examined by scanning electron microscopy. The electrochemical response of the bioanode array demonstrated the oxidation of 0.1Â M glucose, leading to a current density increase due to FAD+/FADH reactions. The above procedure was corroborated through scanning electrochemical microscopy, which showed an increase in the current (400Â pA) in the presence of glucose on the bioanode. The paper-based microfluidic fuel cell performance showed power densities of 0.16 and 0.12Â mWÂ cmâ2 using human serum and blood samples, respectively. Furthermore, the stability of the device was measured in terms of the recovery after obtaining four polarization curves that lasted 30Â min, after which the sample dried out. This work represents a great advancement in the integration of a paper-based microfluidic fuel cell towards autonomous lateral flow assays.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Electrochemistry
Authors
A. Dector, J. Galindo-de-la-Rosa, D.M. Amaya-Cruz, A. OrtÃz-VerdÃn, M. Guerra-Balcázar, J.M. Olivares-RamÃrez, L.G. Arriaga, J. Ledesma-GarcÃa,