Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7715045 | International Journal of Hydrogen Energy | 2015 | 12 Pages |
Abstract
Sustainable production of biochemicals from cellulosic biomass is an attractive alternative to chemical production from fossil fuels. We describe the further development of a three protein synthetic pathway to convert NADPH and H+ to hydrogen using a ferredoxin-NADP+ reductase, a ferredoxin, and the [FeFe] hydrogenase from Clostridium pasteurianum at a rate greater than 14Â mmol H2Â Lâ1 hrâ1 using natural enzymes. We also demonstrate the feasibility of coupling this pathway to a cell-free extract to convert glucose to hydrogen in a potentially cost effective manner. Both of these accomplishments serve as the basis for further engineering to optimize both the yield and productivity of a low-cost cell-free process for the production of highly reduced biochemicals.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Electrochemistry
Authors
Franklin Lu, Phillip R. Smith, Kunal Mehta, James R. Swartz,