Optimization of substrate composition for biohydrogen production from buffalo slurry co-fermented with cheese whey and crude glycerol, using microbial mixed culture

The results demonstrate the usefulness of the mixture design for finding the optimal substrate composition, using BS as co-fermentation substrate to obtain high H2 production yields. Moreover the response surface shows the possibility of mixing the substrates in different ways, while maintaining H2 production within an optimum range: 105-117 mL H2/g VSadded. This might offer a considerable advantage in the effective management of systems or processes, in which the substrates availability may change over the time.