Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7717826 | International Journal of Hydrogen Energy | 2014 | 9 Pages |
Abstract
The microbial community structure of thermophilic mixed culture sludge used for biohydrogen production from palm oil mill effluent was analyzed by fluorescence in situ hybridization (FISH) and 16S rRNA gene clone library techniques. The hydrogen-producing bacteria were isolated and their ability to produce hydrogen was confirmed. The microbial community was dominated by Thermoanaerobacterium species (â¼66%). The remaining microorganisms belonged to Clostridium and Desulfotomaculum spp. (â¼28% and â¼6%, respectively). Three hydrogen-producing strains, namely HPB-1, HPB-2, and HPB-3, were isolated. 16S rRNA gene sequence analysis of HPB-1 and HPB-2 revealed a high similarity to Thermoanaerobacterium thermosaccharolyticum (98.6% and 99.0%, respectively). The Thermoanaerobacterium HPB-2 strain was a promising candidate for thermophilic fermentative hydrogen production with a hydrogen yield of 2.53Â mol H2 molâ1hexose from organic waste and wastewater containing a mixture of hexose and pentose sugars. Thermoanaerobacterium species play a major role in thermophilic hydrogen production as confirmed both by molecular and cultivation-based analyses.
Keywords
Related Topics
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Chemistry
Electrochemistry
Authors
Supachai Nitipan, Chonticha Mamimin, Nugul Intrasungkha, Nils KÃ¥re Birkeland, Sompong O-Thong,