Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7718172 | International Journal of Hydrogen Energy | 2014 | 7 Pages |
Abstract
A unique thermophilic fermentative hydrogen-producing strain H53214 was isolated from a deep-sea hydrothermal vent environment, and identified as Caloranaerobacter azorensis based on bacterial 16S rRNA gene analysis. The optimum culture condition for hydrogen production by the bacterium, designated C. azorensis H53214, was investigated by the response surface methodology (RSM). Eight variables including the concentration of NaCl, glucose, yeast, tryptone, FeSO4 and MgSO4, initial pH and incubation temperature were screened based on the Plackett-Burman design. The results showed that initial pH, tryptone and yeast were significant variables, which were further optimized using the steepest ascent method and Box-Behnken design. The optimal culture conditions for hydrogen production were an initial pH of 7.7, 8.3 g Lâ1 tryptone and 7.9 g Lâ1 yeast. Under these conditions, the maximum cumulative hydrogen volume, hydrogen yield and maximum H2 production rate were 1.58 L H2 Lâ1 medium, 1.46 mol H2 molâ1 glucose and 25.7 mmol H2 gâ1 cell dry weight (CDW) hâ1, respectively. By comparison analysis, strain H53214 was superior to the most thermophilic hydrogen producers because of the high hydrogen production rate. In addition, the isolation of C. azorensis H53214 indicated the deep-sea hydrothermal environment might be a potential source for fermentative hydrogen-producing thermophiles.
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Authors
Lijing Jiang, Chuannan Long, Xiaobing Wu, Hongxiu Xu, Zongze Shao, Minnan Long,