Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7718915 | International Journal of Hydrogen Energy | 2014 | 12 Pages |
Abstract
Two continuous anaerobic fluidized bed reactors (AFBRs) were operated under thermophilic (55 °C) temperature for 150 days to investigate the effect of dark H2 fermentation of diluted and raw sugarcane vinasse on H2 production using mixed seed sludge. Although effective H2 production (52.8% of H2; 0.80 L H2 hâ1 Lâ1; 0.79 mmol gCODaddedâ1) was observed using an elevated substrate concentration (30,000 mg COD Lâ1), the optimal operational conditions were found for the AFBR1 (10,000 mg COD Lâ1) fed with diluted sugarcane vinasse (HRT 6 h; OLR 40 kg COD mâ3 dâ1), achieving a H2 yield of 2.86 mmol H2 g CODaddedâ1. H2 production was inhibited by elevated volatile fatty acid (VFA) concentrations (butyric and acetic acids = 3.7 and 3.0 g Lâ1, respectively) in the raw feedstock. Denaturing gradient gel electrophoresis (DGGE) analyses revealed changes in the bacterial population of the expanded clay biofilm as a function of the substrate concentration.
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Authors
Samantha Christine Santos, Paula Rúbia Ferreira Rosa, Isabel Kimiko Sakamoto, Maria Bernadete Amâncio Varesche, Edson Luiz Silva,