Inhibiting the leaching of lipase from mesoporous supports by polymerization of grafted vinyl groups

This communication reports a method to prevent leaching of porcine pancreatic lipase (PPL) from the pore channels of SBA-15, whereby the pore size is reduced by chemical modification after uptake of porcine pancreatic lipase (PPL). The concrete procedure involves silylation of the surface of SBA-15 entrapping PPL with 3-(trimethoxysilyl)-propylmethacrylate (H2CC(CH3)CO2(CH2)3Si(OCH3)3, PMA) followed by polymerization of the pendant vinyl groups and free PMA, initiated by 2,2′-azobisisobutyronitrile (AIBN).