Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7809666 | Journal of Molecular Structure | 2015 | 29 Pages |
Abstract
This study deals with developing bentonite-tryptophan (Bent-Trp) microcomposite affinity sorbent (38-105 μm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent-Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3 μmol gâ1 bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16 mg/g at pH 10.0 and 1.25 mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21 mg gâ1 sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Tulden Kalburcu, Ahmet Tabak, Nevra Ozturk, Nalan Tuzmen, Sinan Akgol, Bulent Caglar, Adil Denizli,