Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8114 | Biomaterials | 2011 | 6 Pages |
Abstract
Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe3O4 nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Souvik Biswas, Laura E. Gordon, Geoffrey J. Clark, Michael H. Nantz,