Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8251366 | Radiation Physics and Chemistry | 2018 | 8 Pages |
Abstract
Black ginseng extract (BGE) was irradiated at various doses (10, 30, 50, 70, and 100â¯kGy) to improve its biological activity. The content of reducing sugar was significantly increased by gamma irradiation up to 100â¯kGy. The gamma-irradiated BGE showed its highest anti-oxidant activity (DPPH radical scavenging activity-IC50 = 4.33â¯Âµg, ABTS radical scavenging activity-IC50 = 2.39â¯Âµg, FRAP AEAC = 6.46), and mushroom tyrosinase inhibition activity (IC50 = 12.46â¯mg) when irradiated at the dose of 100â¯kGy. The gamma-irradiated BGE showed a greater inhibitory effect on IBMX-induced melanin synthesis (121.43%), intracellular tyrosinase activity (91.80%), melanogenic enzyme expression (tyrosinase, TRP-1, and TRP-2), as well as H2O2-induced reactive oxygen species production, than was observed with the non-irradiated BGE-treated group (melanin synthesis-146.21% and intracellular tyrosinase activity-117.11%) in B16F10 melanoma cells without cytotoxicity. In addition, we also found that the anti-melanogenic effect of gamma-irradiated BGE is mediated by the cyclic AMP (cAMP) signaling pathway (MITF and CREB), ERK, and p38 MAPK signaling pathway. Our findings suggest that gamma-irradiated BGE could be a potential anti-melanogenic agent for use in skin-whitening cosmetics. Consequently, gamma irradiation could become an effective tool for developing functional foods and cosmetics.
Keywords
AEAC2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acidTRP-2TRP-1MITFCREBERKFRAPABTSDPPHIBMX2,2-diphenyl-1-picrylhydrazyl3-isobutyl-1-methylxanthinecAMPp38 MAPKCyclic adenosine monophosphateascorbic acid equivalent antioxidant capacitycAMP response element binding proteinp38 mitogen-activated protein kinasesferric reducing antioxidant powerextracellular regulated kinase
Related Topics
Physical Sciences and Engineering
Physics and Astronomy
Radiation
Authors
Ha-Yeon Song, Hye-Min Kim, Woo Sik Kim, Eui-Hong Byun, Beom-Su Jang, Dae Seong Choi, Eui-Baek Byun,