Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8262103 | Experimental Gerontology | 2018 | 12 Pages |
Abstract
Decrease in multiple functions occurs in the brain with aging, all of which can contribute to age-related cognitive and locomotor impairments. Brain atrophy specifically in hippocampus, medial prefrontal cortex (mPFC), and striatum, can contribute to this age-associated decline in function. Our recent metabolomics analysis showed age-related changes in these brain regions. To further understand the aging processes, analysis using a proteomics approach was carried out. This study was conducted to identify proteome profiles in the hippocampus, mPFC, and striatum of 14-, 18-, 23-, and 27-month-old rats. Proteomics analysis using ultrahigh performance liquid chromatography coupled with Q Exactive HF Orbitrap mass spectrometry identified 1074 proteins in the hippocampus, 871 proteins in the mPFC, and 241 proteins in the striatum. Of these proteins, 97 in the hippocampus, 25 in mPFC, and 5 in striatum were differentially expressed with age. The altered proteins were classified into three ontologies (cellular component, molecular function, and biological process) containing 44, 38, and 35 functional groups in the hippocampus, mPFC, and striatum, respectively. Most of these altered proteins participate in oxidative phosphorylation (e.g. cytochrome c oxidase and ATP synthase), glutathione metabolism (e.g. peroxiredoxins), or calcium signaling pathway (e.g. protein S100B and calmodulin). The most prominent changes were observed in the oldest animals. These results suggest that alterations in oxidative phosphorylation, glutathione metabolism, and calcium signaling pathway are involved in cognitive and locomotor impairments in aging.
Keywords
GSTP1SOD1S100BCamk2aGrin2aN-methyl d-aspartatePSMAGCGrin2BGNASHRASNeurograninLFQMtCO1Glutathione S-transferase PNRGNPRDX2NDUFS4PRDX3PRDX5Prdx1Cox4i1MPFCNMDAFDRUHPLCNCES.E.M.HCDLC-MS3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonateLC-MS/MSE-valueExpectation valuenormalized collision energyanalysis of the varianceANOVAstandard error of the meanKEGG یا Kyoto Encyclopedia of Genes and Genomes Kyoto Encyclopedia of Genes and GenomesCytochrome C oxidase subunit 1AgingLiquid chromatography-mass spectrometryLiquid chromatography-tandem mass spectrometrymedial prefrontal cortexBrainLabel-free quantificationfalse discovery rateGene ontologypeptide spectrum matchPeroxiredoxin-1Proteomicsperoxiredoxin-2CHAPSCalmodulinUltrahigh performance liquid chromatography
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Ageing
Authors
Hamizah Shahirah Hamezah, Lina Wati Durani, Daijiro Yanagisawa, Nor Faeizah Ibrahim, Wan Mohd Aizat, Jean Pierre Bellier, Suzana Makpol, Wan Zurinah Wan Ngah, Hanafi Ahmad Damanhuri, Ikuo Tooyama,