Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8287498 | Acta Histochemica | 2018 | 11 Pages |
Abstract
PLA enabled highly specific detection of M3 receptor expression, demonstration of M3/GP differential coupling and intracellular M3 trafficking in human detrusor smooth muscle cells. This new approach minimized background fluorescence and antibody cross-reactions resulting from single antibody application, and enhanced specificity due to the use of two primary antibodies. Use of subcellular markers allowed visualization of subcellular receptor location. PLA/CLSM allows analyses of muscarinic “receptor - G protein - promiscuity” and intracellular trafficking even in bladder paraffin sections and may give new insights into the etiology and pathology of BPS/IC.
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Authors
Mandy Berndt-Paetz, Luise Herbst, Annett Weimann, Andreas Gonsior, Jens-Uwe Stolzenburg, Jochen Neuhaus,