Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8288485 | Archives of Biochemistry and Biophysics | 2018 | 41 Pages |
Abstract
Mannose-binding lectin (MBL)-Associated Serine Proteases (MASP)-1 and 3, key enzymes in the lectin complement pathway of innate immune response, are also expressed in glioma cell lines. We investigated MASP-1 and MASP-3 expression during dibutyryl cyclic AMP (dbcAMP)- or Interleukin-6 (rIL-6)-induced astrocytic differentiation of C6 glioma cells. Our results demonstrate that C6 cells express basal levels of MASP-1 and MASP-3 and following exposure to dbcAMP or IL-6, a consistent MASP-1 and MASP-3 mRNA up-regulation was found, with a behavior similar to that showed by the fibrillary acidic protein (GFAP). Furthermore, in cell conditioned media, rIL-6 stimulated MASP-3 secretion which reached levels similar to those obtained by dbcAMP treatment. Moreover, the detection of a 46-kDa MASP-3 suggested its processing to the mature form in the extracellular cell medium. Interestingly, the H89 PKA inhibitor, mostly affected dbcAMP-induced MASP-1 and MASP-3 mRNA levels, compared to that of rIL-6, suggesting that cAMP/PKA pathway contributes to MASP-1 and MASP-3 up-regulation. MASP-1 and MASP-3 expression increase was concomitant with dbcAMP- or rIL-6-induced phosphorylation of STAT3. Our findings suggest that the increase in intracellular cAMP concentration or rIL-6 stimulation can play a role in innate immunity enhancing MASP-1 and MASP-3 expression level in C6 glioma cells.
Keywords
pKaAstrocytic differentiationLectin complement pathwayMASPLCPdbcAMPGBMSTAT3GAPDHGFAPDMEMIL-6Dulbecco's modified Eagle's mediuminterleukin-6 (IL-6)interleukin-6CNScentral nervous systemsignal transducer and activator of transcription 3Glial fibrillary acidic proteinGlial fibrillary acidic protein (GFAP)protein kinase Aglyceraldehyde-3-phosphate dehydrogenaseGlioblastoma multiforme
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Authors
Valentina Pagliara, Maddalena Parafati, Annagrazia Adornetto, Misti C. White, Mariorosario Masullo, Maurizio Grimaldi, Rosaria Arcone,