Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8288523 | Archives of Biochemistry and Biophysics | 2018 | 8 Pages |
Abstract
We observed the effects of naringin on bone loss in glucocorticoid-treated inflammatory bowel disease (IBD) in a rat model. The IBD model was established in Sprague-Dawley rats by administering 5.0% dextran sodium sulfate. Dexamethasone (DEX) and naringin were given at the second week. Blood, colon and bone samples were collected for biomarker assay, histological analysis or microCT analysis. Superoxide dismutase, catalase and malonaldehyde were measured in bone. A significant decrease of procollagen type 1â¯N-terminal propeptide (P1NP) level was observed in DEX-treated IBD groups compared with the control (pâ¯<â¯0.05). P1NP levels were dose-dependently increased in the presence of naringin intervention. Bone loss and decreased bone biomechanical properties were observed in DEX-treated IBD rats compared with control rats (pâ¯<â¯0.01). Naringin intervention protected against bone loss and decreased bone biomechanical properties. Bone formation related gene mRNA expressions were significantly decreased in DEX-treated IBD rats compared with control rats. Naringin administration reversed the down-regulation of the expressions of those genes. Naringin treatment reduced the oxidative stress in bone from DEX-treated IBD rats. Our data indicated that naringin may have great potential for the treatment of bone loss in glucocorticoid-treated IBD patients via blocking oxidative stress and promoting bone formation.
Keywords
DLSTNFMDATRAcP5bTb.NTb.SpTb.ThP1NPDAITRAPIBDRT-PCRCATRANKLDEXDSSSLSBV/TVALPAlkaline phosphataseTartrate-resistant acid phosphatase 5btartrate-resistant acid phosphataseinterleukininflammatory bowel diseasesInflammatory bowel diseaseBone formationOxidative stresstumor necrotic factortrabecular separationDexamethasoneSODbone surfacedextran sodium sulfateSuperoxide dismutasedisease activity indextrabecular numbertrabecular thicknessmalonaldehydeNaringinHematoxylin and Eosinreverse transcription polymerase chain reactionOsteoporosisCatalase (CAT)Glucocorticoid
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Authors
Chengli Li, Jun Zhang, Fang Lv, Xingtao Ge, Gang Li,