Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8288572 | Archives of Biochemistry and Biophysics | 2018 | 13 Pages |
Abstract
According to our results, glycated insulin presented altered conformation and more exposed hydrophobic patches than insulin. Formation of oligomeric species and advanced glycated end products (AGEs) were determined. Lower cell viability, higher apoptosis, and more ROS were detected upon treatment of cells with glycated insulin. Finally, glycated insulin led to increased Lucifer yellow and FITC-dextran transportation across the BBB model which could result from ROS producing and apoptosis-inducing activities of AGE-insulin.
Keywords
TFAAGE productsFITC-dextranT2DMRAGEIGF1RPMSFHUVECRP-HPLCPVDFPBSDMEMFBS1-anilinonaphthalene-8-sulfonateAO/EBDMSODulbecco's modified Eagle MediumMTTROSacridine orange/ethidium bromideTrifluoroacetic acidAlzheimer's diseasestandard error of meanCNSpolyvinylidene difluorideType 2 diabetesType 2 diabetes mellitusDimethyl sulfoxideANSBlood–brain barrierBBBfetal bovine serumHuman umbilical vein endothelial cellsAgecentral nervous systemphosphate buffer salinephenylmethylsulphonyl fluoridelucifer yellowBlood-brain barrierSEMApparent permeabilityPermeabilityPappPETPolyethylene terephthalatereversed-phase high performance liquid chromatographyReactive oxygen speciesinsulin receptorReceptor for advanced glycation end productsinsulin-like growth factor 1 receptor
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Authors
Leila Shahriyary, Gholamhossein Riazi, Mohammad Reza Lornejad, Mansoureh Ghezlou, Bahareh Bigdeli, Behdad Delavari, Fatemeh Mamashli, Shayan Abbasi, Jamshid Davoodi, Ali Akbar Saboury,