Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8288582 | Archives of Biochemistry and Biophysics | 2018 | 21 Pages |
Abstract
This study investigated the effects of a quercetin-supplemented diet on the biochemical changes installed in the heart of NO-deficient rats in terms of oxidants production and NO bioavailability determinants. Sprague-Dawley rats were subjected to NÏ-nitro-l-arginine methyl ester (l-NAME) treatment (360â¯mg/L l-NAME in the drinking water, 4â¯d) with or without supplementation with quercetin (4â¯g/kg diet). l-NAME administration led to increased blood pressure (BP) (30%), decreased nitric oxide synthase (NOS) activity (50%), and increases in NADPH oxidase (NOX)-dependent superoxide anion production (60%) and p47phox protein level (65%). The co-administration of quercetin prevented the increase in BP and the activation of NOX but did not modify the decrease in NOS activity caused by l-NAME. In addition, quercetin affected oxidative stress parameters as glutathione oxidation, and the activities of oxidant detoxifying enzymes superoxide dismutase, glutathione peroxidase, and catalase. Thus, quercetin administration counteracts l-NAME effects on NO bioavailability determinants in vivo, essentially through controlling NOX-mediated superoxide anion production.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Valeria Calabró, MarÃa C. Litterio, Cesar G. Fraga, Monica Galleano, Barbara Piotrkowski,