| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8289119 | Archives of Biochemistry and Biophysics | 2016 | 10 Pages |
Abstract
We have developed a procedure for in-vitro flavinylation of recombinant human apo-SDHA, immobilized on Ni-IMAC resin by a His tag, in a chemically defined medium. In this system flavinylation has a pH optimum of 6.5 and is completely dependent on added SDH5. The results suggest that FAD interacts noncovalently with SDHA in the absence of SDH5. This system will be useful in understanding the process of flavinylation of SDHA and the role of SDH5 in this process.
Keywords
flavin adenine dinucleotide2-MEOD600RFIRCFIMACSDHIPTG2-mercaptoethanolpost-translational modificationSDS-PAGEpolyacrylamide gel electrophoresis in sodium dodecyl sulfateisopropyl β-D-1-thiogalactopyranosidesuccinate dehydrogenaserelative fluorescence intensityAssembly factorElectron transfer complexrelative centrifugal forceimmobilized metal affinity chromatography
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Authors
Lala Zafreen, Nancy Walker-Kopp, Li-Shar Huang, Edward Berry,