Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8289945 | Archives of Biochemistry and Biophysics | 2015 | 6 Pages |
Abstract
Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.
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Authors
John Butler, Neil Smyth, Robert Broadbridge, Claire E. Council, Anthony G. Lee, Claire J. Stocker, David C. Hislop, Jonathan R.S. Arch, Michael A. Cawthorne, J. Malcolm East,