Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8290056 | Archives of Biochemistry and Biophysics | 2014 | 9 Pages |
Abstract
Na+/Mg2+ exchanger plays an important role in cardiovascular system, but the molecular mechanisms still largely remain unknown. The Solute Carrier family 41A1 (SLC41A1), a novel Mg2+ transporter, recently was found to function as Na+/Mg2+ exchanger, which mainly regulates the intracellular Mg2+ ([Mg2+]i) homeostasis. Our present studies were designed to investigate whether SLC41A1 impacts on the fibrogenesis of cardiac fibroblasts under Ang II stimulation. Our results showed that quinidine, a prototypical inhibitor of Na+/Mg2+ exchanger, inhibited Ang II-induced cardiac fibrosis via attenuating the overexpression of vital biomarkers of fibrosis, including connective tissue growth factor (CTGF), fibronectin (FN) and α-smooth muscle actin (α-SMA). In addition, quinidine also decreased the Ang II-mediated elevation of concentration of intracellular Ca2+ ([Ca2+]i) and extrusion of intracellular Mg2+. Meanwhile, silencing SLC41A1 by RNA interference also impaired the elevation of [Ca2+]i, [Mg2+]i efflux and the upregulation of CTGF, FN and α-SMA provoked by Ang II. Furthermore, we found that Ang II-mediated activation of NFATc4 translocation decreased in SLC41A1-siRNA cells. These results support the notion that rapid extrusion of intracellular Mg2+ is mediated by SLC41A1 and provide the evidence that the intracellular free Ca2+ concentration is influenced by extrusion of intracellular Mg2+ which facilitates fibrosis reaction in cardiac fibroblasts.
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Authors
Na Yu, Jianmin Jiang, Yang Yu, Hong Li, Xiaoyang Huang, Yunzi Ma, Luankun Zhang, Jian Zou, Boyu Zhang, Shaorui Chen, Peiqing Liu,