Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8291098 | Archives of Biochemistry and Biophysics | 2012 | 6 Pages |
Abstract
⺠Described is a protocol for producing antibody scFvs using mammalian expression of IgG-like constructs. ⺠scFv material produced in CHO vs. E. coli is contrasted. ⺠A soluble and stable misfolded scFv that demonstrates both specific and non-specific binding was identified.
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Authors
Michelle C. Vendel, Michael Favis, William B. Snyder, Flora Huang, Allan D. Capili, Jianying Dong, Scott M. Glaser, Brian R. Miller, Stephen J. Demarest,