Characterisation of the Nâ²1 isoform of the cyclic AMP-dependent protein kinase (PK-A) catalytic subunit in the nematode, Caenorhabditis elegans

Article ID	Journal	Published Year	Pages	File Type
8291386	Archives of Biochemistry and Biophysics	2012	8 Pages	PDF

Abstract

âº Nâ²1-isoforms of Caenorhabditis elegans PK-A catalytic subunits are N-myristoylatable. âº The presence of myristoyl-glycine has been confirmed by MALDI-TOF MS. âº Protein with C. elegans N-terminus and murine C-terminus has been prepared. âº Myristoylation has little effect on the catalytic properties of the chimera. âº Myristoylated chimera has greater apolar targeting than murine protein.

Keywords

N-myristoylation Isoform Recombinant Alternative splicing Nematode

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

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Characterisation of the Nâ²1 isoform of the cyclic AMP-dependent protein kinase (PK-A) catalytic subunit in the nematode, Caenorhabditis elegans

Authors

Roger A. Clegg, Laura C. Bowen, Annalise V. Bicknell, Mohammad Tabish, Mark C. Prescott, Huw H. Rees, Michael J. Fisher,