UMP kinase from Mycobacterium tuberculosis: Mode of action and allosteric interactions, and their likely role in pyrimidine metabolism regulation

► Mycobacterium tuberculosis pyrH-encoded uridine 5′-monophosphate kinase (MtUMPK) is a tetramer. ► MtUMPK catalyzes phosphorylation of UMP to UDP, using ATP-Mg2+ as phosphate donor. ► MtUMPK exhibits positive cooperative kinetics towards ATP. ► GTP and UTP are, respectively, positive and negative effectors of MtUMPK. ► MtUMPK follows a sequential ordered mechanism, in which ATP binds first.