Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8291980 | Archives of Biochemistry and Biophysics | 2008 | 7 Pages |
Abstract
To investigate the role of integrin and G protein pathways in the mechanotransduction process within MCCs and explore the possible crosstalk between the two traditional signal pathways, in vitro-cultured rabbit MCCs were treated with pressure. The mRNA level of α5β1 integrin was determined by in situ hybridization and the distributions of vinculin, Gαq/11 protein, F-actin and intracellular calcium were studied with a laser scanning confocal microscope. Increased integrin α5β1 expression, enhanced stress fiber assembly, elevated G protein and vinculin level and up-regulated IP3 channel sensitivity were found in the mechanotransduction process of MCCs under pressure. Furthermore, the vinculin and the Gαq/11 were observed co-localized with each other, and the F-actin reassembly and stress fibers formation could be inhibited by intracellular calcium channel blocking, which gave direct evidence that the traditional integrin-mediated or G protein-mediated signaling pathways coordinately regulate the function of MCCs under mechanical stimulation.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Min Zhang, Yong-Jin Chen, Takahiro Ono, Jing-Jie Wang,