Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8292270 | Biochemical and Biophysical Research Communications | 2018 | 6 Pages |
Abstract
Intracellular protein delivery is of significance for cellular protein analysis and therapeutic development, but remains challenging technically. Herein, we report a general and highly potent strategy for intracellular protein delivery based on commercially available cationic lipids. In this strategy, a designed double branching poly-glutamate tag is site-specifically attached onto the C-terminal of protein cargos via expressed protein ligation (EPL), which mediates the entrapment of proteins into cationic liposomes driven by electrostatic interaction. The resultant protein-lipid complexes can enter into cytosol with a high efficiency even at the low protein concentration while maintaining protein's biological activity.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Si-Qi Huang, Bei-Bei Han, Yan-Mei Li, Yong-Xiang Chen,