Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8292406 | Biochemical and Biophysical Research Communications | 2018 | 5 Pages |
Abstract
Adenosine kinase (ADK) plays an important role in the growth and development of organisms. A convenient, quick, reliable, sensitive and low-cost assay for ADK activity is of great significance. Here, we found the reaction system with bromothymol blue as the pH indicator had a maximum absorption peak at 614â¯nm. The absorbance change in 614â¯nm was positively correlated with the generated hydrogen ions in the reaction catalyzed by ADK. Then, we demonstrated this assay was feasible for ADK activity. Further, we analyzed the effects of buffer, bromothymol blue concentrations on the sensitivity of the assay, and investigated the sensitivity of ADK contents and adenosine concentration on the assay. Finally, we calculated the Km and Vmax of ADK from Bombyx mori with this assay. Our results suggested this assay was quick, convenient, reliable, sensitive and economic for the activity of ADK. It is an excellent alternative for the conventional ADK assays.
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Authors
Yu Li, Yejing Wang, Kai Song, Lina Liu, Rui Cai, Gang Tao, Ping Zhao, Haimeng Zhou, Huawei He,