Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8292546 | Biochemical and Biophysical Research Communications | 2018 | 7 Pages |
Abstract
Oxidative stress, which can be caused by an overproduction of reactive oxygen species (ROS), often leads to cell death. In recent years, c-Jun NH2-terminal kinase (JNK)-associated leucine zipper protein (JLP, also known as SPAG9 or JIP4), a scaffold protein for JNK mitogen-activated protein kinase (MAPK) signaling pathways, was found to serve as a novel biomarker for cancer. However, although JNK MAPK pathways are reported to be activated in response to various stimuli, including oxidative stress, whether JLP is involved in ROS signaling remains unknown. In this study, we examined the role of JLP in hydrogen peroxide (H2O2)-induced cancer cell death, and found that JLP knockdown (KD) cells exhibit a substantially enhanced cell death response, along with increased intracellular ROS levels. This is the first demonstration of a protective role for JLP in response to cell-death stimulation. We also found that the H2O2-induced JNK activation was attenuated in JLP KD cancer cells. The decreases in cell viability and JNK activation in the JLP KD cells were almost completely reversed by expressing wild-type JLP, but not a mutant JLP lacking the JNK-binding domain. These data collectively suggest that the JLP-JNK signaling pathway counteracts ROS-induced cancer cell death.
Keywords
shRNAJBDJLPc-Jun NH2-terminal kinasePARPGSTFBSJnkPBSHCCMAPKshort hairpin RNAROSHydrogen peroxideOxidative stressdihydroethidiumfetal bovine serumPhosphate buffered salineCell deathKnockdownhemagglutininH2O2DHEpolymerase chain reactionPCRScaffold proteinmitogen-activated protein kinasePropidium iodidePoly(ADP-ribose) polymeraseHepatocellular carcinomaglutathione S-transferaseReactive oxygen species
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Authors
Rong Li, I. Ketut Gunarta, Ryusuke Suzuki, Jambaldorj Boldbaatar, Ryota Nakazato, Dewi Yuliana, Gantulga Davaakhuu, Tsendsuren Oyunsuren, Nobuhiko Takamatsu, Masahiko Kobayashi, Atsushi Hirao, Katsuji Yoshioka,