Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8292720 | Biochemical and Biophysical Research Communications | 2018 | 5 Pages |
Abstract
Muscle cells secrete numerous molecules that function as endocrine hormones and regulate the functions of distant organs. Myelination in the central nervous system (CNS) is regulated by peripheral hormones. However, the effects of muscle-derived molecules on myelination have not been sufficiently analyzed. In this study, we show that muscle-releasing factors promote proliferation of oligodendrocyte precursor cells (OPCs), which is an element of myelination process. Supernatants of mouse myotube cultures stimulated bromodeoxyuridine (BrdU) incorporation into mouse OPCs. Mouse myotube supernatants did not enhance mouse OPC transmigration and myelin basic protein (MBP) expression. RNA sequencing identified candidate genes with hormonal functions that were expressed in mouse myotubes. These data support the possibility that hormonal molecules secreted by myotubes contribute to OPC proliferation and myelination.
Keywords
PBSPFAOPCDAPIbFGFMBPPDGFFBSDMEMIGFPLL4′,6-diamidino-2-phenylindole5-bromo-2′-deoxyuridineBDNFBSADulbecco's modified Eagle Mediumbovine serum albuminaxoninterleukinBrdUCNSfetal bovine serumoligodendrocyte precursor cellcentral nervous systemNeural networkSpinal cordplatelet-derived growth factorbasic fibroblast growth factorInsulin-like growth factorBrain-derived neurotrophic factorPhosphate buffered salineBrainMultiple sclerosisNeuronparaformaldehydeMyelin basic proteinPoly-l-lysine
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Authors
Akino Nakasone, Rieko Muramatsu, Yuki Kato, Yukio Kawahara, Toshihide Yamashita,