Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8292791 | Biochemical and Biophysical Research Communications | 2018 | 5 Pages |
Abstract
In this study, we established an in vitro hypoxic system driven by a self-regulated chemical reaction that proved effective for cell culture. The hypoxic device was modified from a 1.5â¯L polypropylene preservation box normally employed for food storage. Pyrogallic acid, sodium hydroxide, and sodium carbonate were dissolved in water and injected into the box. Sodium dihydrogen phosphate solution was injected into the box after 15â¯min. We measured the concentrations of oxygen and carbon dioxide in the box to determine viability of the hypoxic system. It maintained low levels of oxygen less than 0.2% and stabilizing levels of carbon dioxide at 5% for at least 96â¯h. Therefore, this device sustained a stable hypoxic environment that may be applicable for cell culture and in vitro studies of hypoxia.
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Authors
Ning Han, Zhaotao Wang, Guoqi Wang, Jun Yu, Chen Chen, Huiyong Huang, Ruxiang Xu,