Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8293994 | Biochemical and Biophysical Research Communications | 2018 | 6 Pages |
Abstract
Unbalanced DNA mixture is still a difficult problem for forensic practice. DIP-STRs are useful markers for detection of minor DNA but they are not widespread in the human genome and having long amplicons. In this study, we proposed a novel type of genetic marker, termed DIP-SNP. DIP-SNP refers to the combination of INDEL and SNP in less than 300bp length of human genome. The multiplex PCR and SNaPshot assay were established for 14 DIP-SNP markers in a Chinese Han population from Shanxi, China. This novel compound marker allows detection of the minor DNA contributor with sensitivity from 1:50 to 1:1000 in a DNA mixture of any gender with 1â¯ng-10â¯ng DNA template. Most of the DIP-SNP markers had a relatively high probability of informative alleles with an average I value of 0.33. In all, we proposed DIP-SNP as a novel kind of genetic marker for detection of minor contributor from unbalanced DNA mixture and established the detection method by associating the multiplex PCR and SNaPshot assay. DIP-SNP polymorphisms are promising markers for forensic or clinical mixture examination because they are shorter, widespread and higher sensitive.
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Biochemistry
Authors
Zhizhen Liu, Jinding Liu, Jiaqi Wang, Deqing Chen, Zidong Liu, Jie Shi, Zeqin Li, Wenyan Li, Gengqian Zhang, Bing Du,