Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8294233 | Biochemical and Biophysical Research Communications | 2018 | 19 Pages |
Abstract
The circRNAs are differentially expressed in a wide range of cancers in regulating their initiation and progression, and could be used to make a diagnosis for some diseases like tumor as a new biomarker. However, the correlation and the mechanism of action between circRNAs and colorectal cancer (CRC) are still unclear. In this study, by using qRT-PCRs, we detected the expression level of hsa_circ_0001649 in tissue and serum samples from CRC patients, and the cultured cell has been detected. We found that the hsa_circ_0001649 in CRC is significantly lower than the expression level of correspondent nontumorous tissues (nâ¯=â¯64, Pâ¯<â¯0.01). We also tested the HCT116â¯cell lines, and the similar result is observed (nâ¯=â¯15, Pâ¯<â¯0.01). Moreover, we detected the serum samples obtained before and after surgery, showing significantly the expression level of hsa_circ_0001649 in the same patient is up-regulated after surgery (nâ¯=â¯18, Pâ¯<â¯0.01). Besides, we analyzed the correlation between clinicopathological date and the expression level of hsa_circ_0001649, we found that hsa_circ_0001649 expression level is closely associated with pathological differentiation (Pâ¯=â¯0.037), and the result also illustrated that the expression level of hsa_circ_0001649 is no direct correlation with age, gender, TMN stage, lymphatic metastasis, CEA, CA19-9, and CA-724 levels. The area under the receiver operating characteristic (ROC) curve was 0.857. In conclusion, this study showed that the expression level of hsa_circ_0001649 was down-regulated in CRC and could use it as a new biomarker for specific and sensitive inspection of CRC.
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Authors
Wenxin Ji, Chunli Qiu, Mao Wang, Ning Mao, Shaofeng Wu, Yinhai Dai,