Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8294299 | Biochemical and Biophysical Research Communications | 2018 | 23 Pages |
Abstract
T-Cell Lymphoma Invasion and Metastasis 1 (Tiam1) is a specific nucleotide exchange factor (GEF) that can activate Rho-like GTPase and Rac1 and regulate various cellular processes, including cell cycle progression and cell migration. The roles of Tiam1 in vascular intimal hyperplasia, especially in vascular smooth muscle cell proliferation and migration, are not fully understood. In this study, we investigated the effect of Tiam1 on vascular intimal hyperplasia in a carotid artery ligation model and human aortic smooth muscle cells (HASMCs). We found that the expression of Tiam1 was up-regulated in the neointima of carotid artery ligation mice and that Tiam1â/â mice following carotid artery ligation had less neointimal formation compared with wild type mice. Knockdown of Tiam1 by siRNA markedly attenuated PDGF-induced migration and proliferation in HASMCs by inhibiting the activation of Rac1. Therefore, these results suggest that Tiam1 is an important regulator of intima hyperplasia. It may regulate vascular intimal hyperplasia through the activation of Rac1.
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Authors
Wenxin Kou, Xu Xu, Shuya Ji, Mengyao Chen, Dongdong Liu, Kai Wang, Jianhui Zhuang, Qing Yu, Qian Zhao, Yawei Xu, Hongli Zhang, Wenhui Peng,