Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8294952 | Biochemical and Biophysical Research Communications | 2018 | 7 Pages |
Abstract
The gene therapy of cancer, due to the limit of its efficiency and safety, has not been widely used in clinical. Recently, bacterial magnetic particles (BMPs), which are membrane-bound nanocrystals found in magnetotactic bacteria, have been exploited as a new gene delivery system. However, its application on gene therapy remains to be explored. In our previous study, we found that a combination of cecropin B (ABPs) and apoptin (VP3) could serve as an effective gene therapeutic agent. Thus, in this study, we used BMPs to deliver the co-expression plasmid of these two gene, namely pVAX1-VA, and evaluated its therapeutic effect on human hepatocellular carcinoma (HepG2). Our results showed that BMPs significantly improved the efficiency of gene transfection (almost 3-fold than Lipofectamine 2000â¯at 48â¯h, Pâ¯<â¯.001), which led to stronger apoptosis (in a peak almost 2-fold than Lipofectamine 2000-pVAX1-VA, Pâ¯<â¯.01) and growth inhibition of HepG2 cells. More importantly, compared with Lipofectamine 2000-pVAX1-VA group, BMP-pVAX1-VA strikingly inhibited tumor growth (0.60â¯Â±â¯0.09â¯g vs. 0.88â¯Â±â¯0.11â¯g, Pâ¯<â¯.05) in nude mouse tumor models and increased the tumor-infiltrating lymphocytes considerably without apparent cytotoxicity. These findings suggest that BMPs could be an attractive gene delivery system for gene therapy and provide a potential available treatment for human hepatocellular carcinoma and maybe some other kinds of tumors.
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Authors
Xi Wang, Ji-gui Wang, Yuan-yuan Geng, Jiao-jiao Wang, Xiao-mei Zhang, Shuang-shuang Yang, Wei Jiang, Wei-quan Liu,