Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8295082 | Biochemical and Biophysical Research Communications | 2018 | 27 Pages |
Abstract
Advanced glycation end products (AGEs) are harmful compounds generated by nonspecific glycation of proteins and lipids. The accumulation of AGEs is associated with various diseases, including breast cancer. AGEs have been shown to promote a breast cancer cell line by enhancing proliferation, invasion and migration. In this study, we investigated the effect and associated mechanism of AGEs on triple negative breast cancer cells. AGEs enhanced the proliferation, tumorigenicity, invasion and migration of primary breast cancer cells. AGEs also enhanced the RNA and protein expression of matrix metalloproteinase (MMP)-9 and its gelatinase activity. Enhanced MMP-9 expression was mediated by extracellular-signal regulated kinase (ERK) and nuclear factor kappa B (NF-κB) pathways. Moreover, inhibitors of ERK and NF-κB signaling attenuated the effect of AGEs on tumorigenicity, invasion and migration of primary breast cancer cells. Taken together, we suggest that AGEs directly promote primary breast cancer cells via the ERK and NF-κB pathway, which may lead to advanced therapeutic modalities of breast cancer.
Keywords
Phorbol-12-myristate-13-acetateAGEsERKMMP-9RAGEJnkPBSMMPTNBCNF-κB3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromidec-Jun KinasePMAMAPKMTTsodium dodecyl sulfate-polyacrylamide gel electrophoresisSDS-PAGEInvasionBreast cancerTriple-negative breast cancerextracellular-signal regulated kinasenuclear factor kappa BMatrix metalloproteaseAdvanced glycosylation end productAdvanced glycation end productsPhosphate-buffered salineMigrationmitogen-activated protein kinaseReceptor for advanced glycation end products
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Authors
Kyung Jin Lee, Ji Won Yoo, Yun Kyu Kim, Jae Ho Choi, Tae-Yong Ha, Minchan Gil,