| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8297655 | Biochemical and Biophysical Research Communications | 2013 | 7 Pages |
Abstract
Previous studies have confirmed the therapeutic effects of bone marrow stromal cells (BMSCs) transplantation on cerebral ischemia. However, the proliferative, differentiative, and homing capacity of BMSC from the elderly are significantly reduced, especially after several passages expansion in vitro. In this study, by introducing lentivirus-mediated hTERT and VEGF genes to modify human BMSCs from aged donors, we observed extended lifespan, promoted angiogenic capacity while less enhanced tumorigenicity of the genetically engineering BMSCs. These results therefore suggest that the modification of aged BMSCs by dual expression of hTERT and VEGF may be used for autologous cell replacement for ischemic cerebrovascular disease in elderly patients.
Keywords
FACSTelomerase RNA componenthBMSCTERCPDLTRAPBCAMSChTERTMOIinternal standardbicinchoninic acidEnzyme-linked immunosorbent assayELISAhuman telomerase reverse transcriptasefluorescence-activated cell sortingpopulation doubling levelBone marrow stromal cellMarrow stromal cellVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)Genetic engineeringTelomeric repeat amplification protocolmultiplicity of infection
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Authors
Hao Tang, Yongsheng Xiang, Xiaodan Jiang, Yiquan Ke, Zongyu Xiao, Yang Guo, Qiujing Wang, Mouxuan Du, Linsha Qin, Yuxi Zou, Yingqian Cai, Zhenzhou Chen, Ruxiang Xu,