| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8298006 | Biochemical and Biophysical Research Communications | 2011 | 6 Pages |
Abstract
⺠Chaotropic denaturation of immobilized protein antigen on a microplate is compatible with antibody detection by indirect ELISA. ⺠A short chemical denaturation step can facilitate antibody binding an increase antigen detection limit of an indirect ELISA. ⺠The quantitative detection of protein antigens by indirect ELISA can be improved by a simple chemical denaturation step.
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Authors
Robert Hnasko, Alice Lin, Jeffery A. McGarvey, Larry H. Stanker,