Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8298457 | Biochemistry and Biophysics Reports | 2018 | 6 Pages |
Abstract
Programmed cell death-ligand 1 (PD-L1), which is a ligand of programmed cell death-1 (PD-1), is a type I transmembrane glycoprotein that is expressed on antigen-presenting cells and several tumor cells, including melanoma and lung cancer cells. There is a strong correlation between human PD-L1 (hPD-L1) expression on tumor cells and negative prognosis in cancer patients. In this study, we produced a novel anti-hPD-L1 monoclonal antibody (mAb), L1Mab-4 (IgG2b, kappa), using cell-based immunization and screening (CBIS) method and investigated hPD-L1 expression in oral cancers. L1Mab-4 reacted with oral cancer cell lines (Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4) in flow cytometry and stained oral cancers in a membrane-staining pattern. L1Mab-4 stained 106/150 (70.7%) of oral squamous cell carcinomas, indicating the very high sensitivity of L1Mab-4. These results indicate that L1Mab-4 could be useful for investigating the function of hPD-L1 in oral cancers.
Keywords
ACCCTLA-4HNCcytotoxic T-lymphocyte-associated antigen 4APCPD-L1MECSCCPD-1FBSDABPBSDMEM3,3-diaminobenzidine tetrahydrochlorideBSADulbecco's modified Eagle's mediumbovine serum albuminMonoclonal antibodyantigen-presenting cellEDTAEthylenediaminetetraacetic acidOral cancerHead and neck cancerfetal bovine serumProgrammed cell death-ligand 1Phosphate-buffered salineProgrammed cell death-1Adenoid cystic carcinomaSquamous cell carcinomamucoepidermoid carcinoma
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Authors
Shinji Yamada, Shunsuke Itai, Mika K. Kaneko, Yukinari Kato,