| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8300075 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2012 | 9 Pages |
Abstract
⺠We have prepared a systematic series of fragments of the human Y4 GPCR. ⺠The N terminus of the fragments is fused to Lep or Mistic, and the C terminus to either GFP or PhoA. ⺠GFP fluorescence and PhoA activity are measured to establish the location of the C terminus. ⺠All fragments assume more than one topology, but often one topology predominates. ⺠Two pairs of large complementary fragments are produced, purified, characterized by SEC, and shown to interact.
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Authors
Jacopo Marino, Eric R. Geertsma, Oliver Zerbe,