| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8300105 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2012 | 10 Pages |
Abstract
⺠We monitored the differential membrane behavior of glycine receptor α3 isoforms. ⺠Both ensemble average and single particle fluorimetric techniques were applied. ⺠Aggregation and diffusion were quantified using fixed and live-cell measurements. ⺠Evidence for molecular interaction of isoforms after membrane insertion is revealed. ⺠Considerations for fluorimetric probing of membrane protein behavior are reported.
Keywords
SPTdSTORMHEK 293Raster image correlation spectroscopyICSTIRFMGlyRDichroic mirrorPFAtotal internal reflection fluorescence microscopeFRAPRICSROICLSMTime laganomalous diffusionTlagTicsMean Square Displacementd′Single particleSingle particle trackingImage correlation spectroscopyfluorescence recovery after photobleachingEnsemble averageregion of interestConfocal laser scanning microscopedirect stochastic optical reconstruction microscopyNanoscopyhemagglutininparaformaldehydehuman embryonic kidney 293glycine receptor
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Kristof Notelaers, Nick Smisdom, Susana Rocha, Daniel Janssen, Jochen C. Meier, Jean-Michel Rigo, Johan Hofkens, Marcel Ameloot,