| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8300144 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2011 | 11 Pages |
Abstract
⺠Effect of cholesterol on the organization of plasma membranes and G protein activation. ⺠Cholesterol depletion increases membrane fluidity and mobility of plasma membranes. ⺠Cholesterol depletion does not alter the agonist binding site of δ-opioid receptor (DOR). ⺠Cholesterol depletion decreases the ability of DOR agonist to activate G proteins. ⺠Indication that biophysical state of membrane is a regulatory factor of DOR-signaling cascade.
Keywords
DMEMheterotrimeric guanine nucleotide-binding regulatory proteinsTRH-RThyrotropin-releasing hormone receptorDRMSDPHPTXGPCRPMSFPBSβ-CyclodextrinTCSPCHEKTBS1,6-diphenyl-1,3,5-hexatrieneDulbecco's modified Eagle's mediumδ-Opioid receptortrhTris-buffered salineDADLEDORpertussis toxinTime correlated single photon countingPlasma membranephenylmethylsulfonyl fluoridePhosphate-buffered salinethyrotropin-releasing hormoneG proteinsPNSCholesterol depletionhuman embryonic kidneyG protein-coupled receptor
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Authors
Jana Brejchová, Jan Sýkora, KateÅina Dlouhá, Lenka Roubalová, Pavel OstaÅ¡ov, Miroslava VoÅ¡ahlÃková, Martin Hof, Petr Svoboda,