Expression, purification, electron microscopy, N-glycosylation mutagenesis and molecular modeling of human P2X4 and Dictyostelium discoideum P2XA

Article ID	Journal	Published Year	Pages	File Type
8300158	Biochimica et Biophysica Acta (BBA) - Biomembranes	2011	8 Pages	PDF

Abstract

âº We expressed human P2X4 and Dictyostelium discoideum (Dd) P2XA in Sf9 insect cells. âº Both receptors were capable of binding to ATP-coupled sepharose beads. âº Preparative electrophoresis was used to purify DdP2XA trimers. âº We determined a 21Â Ã-resolution structure of DdP2XA using single particle analysis. âº DdP2XA cannot be modeled accurately using the zebrafish P2X4 crystal structure.

Keywords

Sf9 n-dodecyl-N,N-dimethylamine-N-oxide n-octyl-β-d-glucoside LDAO C12E8 n-dodecylphosphocholine PBS P2X DDM SDS n-Dodecyl-β-D-maltoside N-glycosylation octaethylene glycol monododecyl ether Tem β-OG Single particle analysis sodium dodecyl sulfate Sf9 cells Phosphate-buffered saline Modeller Zebrafish